Optical Rotatory Dispersion of Catalase

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Optical Rotatory Dispersion of Catalase.

It is well recognized that the measurements of optical rotatory dispersion of proteins with strong absorption bands are extremely difficult because of the diminishing intensity of the emergent light. Until very recently, these studies were made possible only by improving the light source and also frequently by a wide opening of the slit width on the monochromator at the expense of spectral puri...

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Quantum optical rotatory dispersion

The phenomenon of molecular optical activity manifests itself as the rotation of the plane of linear polarization when light passes through chiral media. Measurements of optical activity and its wavelength dependence, that is, optical rotatory dispersion, can reveal information about intricate properties of molecules, such as the three-dimensional arrangement of atoms comprising a molecule. Giv...

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Optical rotatory dispersion of nonhelical proteins.

The optical rotatory dispersion of a group of proteins having the Moffitt constant bo near zero was studied in the far ultraviolet spectral zone. Sodium poly-c+L-glutamate, myoglobin, and serum albumin were used as reference substances. It was found that, in addition to the normal type of rotatory dispersion, as displayed, for example, by myoglobin, four other types of curves could be demonstra...

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Optical Rotatory Dispersion and Circular Dichroism of

Studies of optical rotatory dispersion (ORD) and circular dichroism (CD) are reported on pepsinogen, carbamyl-, and succinylpepsinogen. From the ORD measurements in the wave length range of 600 to 300 rnp as functions of urea, temperature, and pH, it was shown that the macromolecular conformation of the three proteins differs markedly. On replacing the e-NH2 groups of the lysine residues of pep...

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Optical Rotatory Dispersion and Conformation of Polyadenylic and Polyuridylic Acids.

The application of optical rot,atory dispersion to nucleic acids and polynucleotides is fairly recent (l-6). Doty et cd. (2) made a comprehensive study of the optical rotatory dispersion of deoxyribonucleic acid, ribonucleic acid, and complexes of some homopolynucleotides; their results strongly suggested a close relationship between the optical rotations and the secondary structure of nucleic ...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1963

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)48656-5